We too have had a couple "ladies of the night".
The first one was a walk in, and it wasn't till after dusk that we discovered what was going on. She wanted to book again the next night but we told her were full. She didn't cause a stink just walked away.
The next one booked online for a week. We never saw her guests coming and going, and she was a he!! She too wanted extra nights and we said sorry.
We did have a few other "suspect" ladies ring not long after she left, and say their friend had just stayed and she wanted too also. We said no sorry, and again lucky for us no worries!!
It is your business and you have a right to say who can and cannot stay at your establishment!